Miniprot Window Spidroin Screen Report - miniprot_window_spidroin_screen_20260526

执行摘要

134

纳入物种

134

运行成功物种

115

有 selected MPID 的物种

1,423

selected MPID 总数

1,083

strong candidates

340

rescue candidates

127

typing 结果存在物种

127

NHMMER 结果存在物种

筛选模式：typing_guided=1423。当前报告只读取已有结果，不重新运行 miniprot、HMMER 或 pyGenomeTracks。

方法概览

流程 QC

step	rows	ok	other
prepare_windows	134	134	0
run_miniprot	134	134	0
split_miniprot	134	134	0
parse_models	134	134	0
select_candidates	134	134	0
export_selected	134	134	0
build_tracks	134	134	0
pygenometracks	0	0	missing

总体结果图表

Positive: min=0.550, median=0.684, max=1.000; coverage: min=0.701, median=1.000, max=1.000; aa length: min=1004, median=3165, max=14282; exon count: min=1, median=4, max=105.

代表性 selected MPID

species	window	MPID	status	type	positive	coverage	aa	exons	target protein
067.Argiope_bruennichi	NC_079157.1_68127848-68456381:.	MP000686	selected_strong	MaSp1	1.0	1.0	7202	1	XP_055944986.1_fibroin_heavy_chain-like_[Argiope_bruennichi]
120.Trichonephila_clavipes	BMAU01021429.1_440510-560960:.	MP000575	selected_strong	AcSp	1.0	1.0	6817	1	GFY34959.1_aciniform_spidroin_1A_variant_1_[Trichonephila_clavipes]
067.Argiope_bruennichi	NC_079157.1_7699452-7842201:.	MP001112	selected_strong	Flag	1.0	1.0	5905	20	XP_055946451.1_fibroin_heavy_chain-like_isoform_X1_[Argiope_bruennichi]
120.Trichonephila_clavipes	BMAU01021438.1_1052740-1168510:.	MP000466	selected_strong	CySp	1.0	1.0	5253	1	GFY36897.1_cylindrical_spidroin_1A_variant_1_[Trichonephila_clavipes]
121.Trichonephila_inaurata_madagascariensis	BMAV01028254.1_1-37243:.	MP000948	selected_strong	MaSp1	1.0	1.0	5063	1	GFS66728.1_major_ampullate_spidroin_1A_variant_1_[Trichonephila_inaurata_madagascariensis]
106.Pardosa_pseudoannulata	Ppse_Chr09_155292767-155425478:.	MP000076	selected_strong	AcSp	1.0	1.0	4323	2	UVM79552.1_aciniform_spidroin_[Pardosa_pseudoannulata]
120.Trichonephila_clavipes	BMAU01021414.1_1-78249:.	MP000336	selected_strong	MaSp1	1.0	1.0	4266	9	GFY33585.1_major_ampullate_spidroin_1A_variant_3_[Trichonephila_clavipes]
071.Caerostris_extrusa	BPLR01021713.1_39823-152533:.	MP000893	selected_strong	MaSp2B	1.0	1.0	4237	1	GIX92710.1_major_ampullate_spidroin_2C_variant_1_[Caerostris_extrusa]
071.Caerostris_extrusa	BPLR01021721.1_72830-156150:.	MP000602	selected_strong	CySp	1.0	1.0	4164	1	GIX92933.1_cylindrical_spidroin_1A_variant_1_[Caerostris_extrusa]
120.Trichonephila_clavipes	BMAU01021436.1_876098-983834:.	MP000056	selected_strong	MaSp	1.0	1.0	4123	1	GFY36414.1_spidroin_1A_variant_1_[Trichonephila_clavipes]
067.Argiope_bruennichi	NC_079157.1_68127848-68456381:.	MP001073	selected_strong	MaSp1	1.0	1.0	4056	1	XP_055943799.1_fibroin_heavy_chain-like_[Argiope_bruennichi]
071.Caerostris_extrusa	BPLR01021715.1_1-155908:.	MP000389	selected_strong	MaSp	1.0	1.0	3989	1	GIX92742.1_major_ampullate_spidroin_1F_variant_1_[Caerostris_extrusa]
121.Trichonephila_inaurata_madagascariensis	BMAV01028241.1_270850-341253:.	MP000836	selected_strong	PySp	1.0	1.0	3780	1	GFS66377.1_pyriform_spidroin_1A_variant_1_[Trichonephila_inaurata_madagascariensis]
067.Argiope_bruennichi	NC_079161.1_38348403-38763164:.	MP001233	selected_strong	AcSp	1.0	1.0	3778	1	XP_055925190.1_fibroin_heavy_chain-like_[Argiope_bruennichi]
120.Trichonephila_clavipes	BMAU01021430.1_228503-339800:.	MP000468	selected_strong	PySp	1.0	1.0	3766	1	GFY35027.1_pyriform_spidroin_1A_variant_1_[Trichonephila_clavipes]
120.Trichonephila_clavipes	BMAU01021427.1_1-143814:.	MP000551	selected_strong	MaSp2	1.0	1.0	3703	5	GFY34690.1_major_ampullate_spidroin_2A_variant_2_[Trichonephila_clavipes]
106.Pardosa_pseudoannulata	Ppse_Chr09_20992074-21103298:.	MP000400	selected_strong	PySp	1.0	1.0	3684	3	UVM79561.1_pyriform_spidroin_[Pardosa_pseudoannulata]
099.Nephila_pilipes	BMAW01132850.1_1-65980:.	MP000403	selected_strong	MaSp2	1.0	1.0	3681	1	GFU45572.1_major_ampullate_spidroin_2A_variant_3_[Nephila_pilipes]
099.Nephila_pilipes	BMAW01132854.1_265406-376498:.	MP000723	selected_strong	MiSp	1.0	1.0	3663	2	GFU45726.1_minor_ampullate_spidroin_1B_variant_2_[Nephila_pilipes]
099.Nephila_pilipes	BMAW01132857.1_91040-151913:.	MP000471	selected_strong	MaSp	1.0	1.0	3625	1	GFU45798.1_major_ampullate_spidroin_2A_variant_2_[Nephila_pilipes]

物种汇总 Top 25

species	status	NHMMER	typing	typing loci	full length loci	selection mode	selected	proteins
126.Araneus_angulatus	ok	present	present	56	49	typing_guided	49	49
067.Argiope_bruennichi	ok	present	present	46	41	typing_guided	42	42
064.Araneus_ventricosus	ok	present	present	45	41	typing_guided	40	40
081.Larinioides_cornutus	ok	present	present	41	38	typing_guided	37	37
063.Araneus_marmoreus	ok	present	present	39	35	typing_guided	33	33
078.Gibbaranea_gibbosa	ok	present	present	34	31	typing_guided	32	32
097.Meta_bourneti	ok	present	present	38	27	typing_guided	32	32
119.Trichonephila_clavata	ok	present	present	39	28	typing_guided	32	32
002.Anyphaena_bomiensis	ok	present	present	33	30	typing_guided	28	28
118.Trichonephila_antipodiana	ok	present	present	33	25	typing_guided	28	28
024.Meta_wanglangensis	ok	present	present	34	27	typing_guided	27	27
082.Larinioides_sclopetarius	ok	present	present	35	30	typing_guided	27	27
120.Trichonephila_clavipes	ok	present	present	34	26	typing_guided	26	26
071.Caerostris_extrusa	ok	present	present	29	27	typing_guided	25	25
108.Salticus_scenicus	ok	present	present	40	30	typing_guided	24	24
033.Peucetia_latikae	ok	present	present	29	25	typing_guided	23	23
086.Leviellus_thorelli	ok	present	present	25	22	typing_guided	23	23
102.Parasteatoda_lunata	ok	present	present	30	24	typing_guided	23	23
098.Metellina_segmentata	ok	present	present	29	26	typing_guided	22	22
121.Trichonephila_inaurata_madagascariensis	ok	present	present	32	21	typing_guided	22	22
106.Pardosa_pseudoannulata	ok	present	present	22	17	typing_guided	21	21
011.Dolomedes_nigrimaculatus	ok	present	present	21	20	typing_guided	20	20
070.Caerostris_darwini	ok	present	present	33	22	typing_guided	20	20
074.Dolomedes_plantarius	ok	present	present	24	18	typing_guided	20	20
090.Lycosa_singoriensis	ok	present	present	25	22	typing_guided	19	19

Selected MPID 示例表

species	window	MPID	status	typing ID	type	positive	identity	coverage	aa	exons	introns
001.Allagelena_difficilis	chr07_71193836-71515633:.	MP000286	selected_strong	Aldi_spid_00005	AcSp	0.6773	0.434	0.9827638342908981	3148	6	5
001.Allagelena_difficilis	chr07_72730900-72857731:.	MP000463	selected_rescue	Aldi_spid_00012	AcSp	0.5514	0.3133	1.0	2477	12	11
001.Allagelena_difficilis	chr07_71193836-71515633:.	MP000604	selected_strong	Aldi_spid_00009	AcSp	0.604	0.3602	1.0	3056	3	2
001.Allagelena_difficilis	chr07_71193836-71515633:.	MP000605	selected_rescue	Aldi_spid_00008	AcSp	0.5973	0.3565	0.9726757747417527	2952	5	4
001.Allagelena_difficilis	chr07_71193836-71515633:.	MP000607	selected_rescue	Aldi_spid_00006	AcSp	0.5913	0.3457	0.9953348883705432	3010	2	1
001.Allagelena_difficilis	chr07_68448877-68572255:.	MP000608	selected_rescue	Aldi_spid_00003	AcSp	0.5619	0.329	1.0	3047	3	2
001.Allagelena_difficilis	chr10_34611983-34727303:.	MP000010	selected_strong	Aldi_spid_00016	CySp	0.7197	0.5614	1.0	4905	2	1
001.Allagelena_difficilis	chr10_35195288-35307698:.	MP000015	selected_strong	Aldi_spid_00017	CySp	0.7976	0.6163	0.9975158708252829	3823	3	2
001.Allagelena_difficilis	chr15_131646135-131753526:.	MP000156	selected_strong	Aldi_spid_00020	MiSp	0.7229	0.592	0.8959381044487428	2351	1	0
001.Allagelena_difficilis	chr15_131276370-131383842:.	MP000321	selected_strong	Aldi_spid_00019	MiSp	0.7427	0.6107	0.9382477807796218	2484	1	0
001.Allagelena_difficilis	chr15_132072043-132185858:.	MP000502	selected_strong	Aldi_spid_00021	MiSp	0.7233	0.6257	0.9455463397042914	2899	5	4
001.Allagelena_difficilis	chr15_132530676-132644902:.	MP000597	selected_strong	Aldi_spid_00022	MiSp	0.7066	0.6023	0.9982554082344731	3038	7	6
001.Allagelena_difficilis	chr15_131149518-131258839:.	MP000599	selected_strong	Aldi_spid_00018	MiSp	0.692	0.5592	0.9986043265875785	2822	3	2
001.Allagelena_difficilis	chr07_218500542-218773467:.	MP000609	selected_strong	Aldi_spid_00013	PySp	0.6673	0.465	1.0	3229	2	1
001.Allagelena_difficilis	chr07_218500542-218773467:.	MP000610	selected_strong	Aldi_spid_00015	PySp	0.6634	0.4618	1.0	3243	3	2
001.Allagelena_difficilis	chr07_218500542-218773467:.	MP000611	selected_strong	Aldi_spid_00014	PySp	0.6629	0.462	1.0	3239	3	2
002.Anyphaena_bomiensis	chr14_79778272-79896991:.	MP002067	selected_strong	Anbo_spid_00033	AcSp	0.6383	0.4292	0.9952952246530228	4690	3	2
002.Anyphaena_bomiensis	chr03_20111972-20287519:.	MP002068	selected_strong	Anbo_spid_00023	AcSp	0.6395	0.4373	0.9656551399670665	4567	2	1
002.Anyphaena_bomiensis	chr01_115094839-115250909:.	MP001397	selected_strong	Anbo_spid_00018	MaSp	0.6076	0.4619	1.0	3768	7	6
002.Anyphaena_bomiensis	chr01_115094839-115250909:.	MP001546	selected_rescue	Anbo_spid_00019	MaSp	0.5772	0.4407	0.9926840420667581	2232	4	3

001 物种 selected gene model 摘要图

从 selected GFF3 抽取 Positive 最高的 mRNA/CDS，绘制简化结构示意；完整坐标与属性请查看 GFF3。

已生成基因组轨道图

已发现 24 张 pyGenomeTracks PNG，本报告嵌入 24 张。

数据产物

name	path
All selected MPID TSV	data/processed/miniprot_window_spidroin_screen_20260526_115407/all_species_selected_spidroin_mpid.tsv
All selected proteins FASTA	data/processed/miniprot_window_spidroin_screen_20260526_115407/all_species_selected_spidroin_proteins.faa
Species run manifest	data/processed/miniprot_window_spidroin_screen_20260526_115407/species_run_manifest.tsv
Interim root	data/interim/miniprot_window_spidroin_screen_20260526_115407

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